777在线视频免费观看,国产真实精品久久二三区,亚洲4438丁香五月天,av片在线观看无码免费

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1676WM-266-4細(xì)胞, 人黑素瘤細(xì)胞

WM-266-4細(xì)胞, 人黑素瘤細(xì)胞

簡要描述:WM-266-4細(xì)胞, 人黑素瘤細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1676
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-06-28
  • 訪  問  量:1837

產(chǎn)品分類

Product Category

詳細(xì)介紹

WM-266-4細(xì)胞, 人黑素瘤細(xì)胞

ATCC® Number:CRL-1676™    Price:$318.00
Designations:WM-266-4Depositors:M HerlynBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth aProperties:adherentOrganism:Homo sapiens (humn)Morphology:epithelial Source:Organ: skin Disease: melanomaCellular Products:proteoglycan antigen characteristic of melanomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.WM-266-4細(xì)胞, 人黑素瘤細(xì)胞Tumorigenic:YesDNA Profile (STR):Amelogenin: X CSF1PO: 12 D13S317: 12,13 D16S539: 11,12 D5S818: 13 D7S820: 8 THO1: 7,9 TPOX: 8,11 vWA: 15,17Cytogenetic Analysis:The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell.Age:58 yearsGender:femaleComments:The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma. Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675).Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol:                                          Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020derived from same individual:ATCC CRL-1675

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
少妇极品熟妇人妻无码| 久久久在线播放视频在线| 亚洲乱码精品97久久久| 按摩AV在线不卡中文网| 农民工猛吸女大学奶头| 男生插女人下面榴莲视频| 日韩电影免费观看一区二区| 九九热这里只有精品26| 啊轻点好爽重磅泄露视频| 尤物视频在线观看网站入口| 啊舒服死了好大插穴视频| 国产成人无码av| 操逼片破处女片| 娇妻与公h喂奶| 初爱视频教程| 日本一区二区三区高清日韩 | 三区 一区 在线 欧美| 国产精品区在线播放vr| 成人黄网在线观看不卡高清| 久久精品亚洲天海翼av| 久久大香香蕉国产免费网| 不用播放器的av| 大鸡巴插的我好爽啊视频| 久久综合给合久久97色| ccyy草草影院国产电影| 美女黄片儿靠逼| 我看大学女生操逼的网站| 男生艹女生网站| 日韩精品日韩视频在线播放| 非洲老太太性爱| 激情无码黄动漫在线观看| av老司机福利精品导航| 亚洲热中文字幕| 五十路レンタのおばさん| 白领人妻系列第26部分阅读| 啊……好爽…再快点动漫| 日韩欧美亚洲国产字幕四区| 国产免费的黄色一级网站| 久久久午夜福利免费影院| 国产剧情三级片在线观看| 国产日韩亚洲欧美国另类|