国产成人无码免费看片软件,亚洲自拍清纯另类国产就,zoz○zo女人和另类zoz0,黑丝美女自慰被大鸡巴操

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2547Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞

Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞

簡(jiǎn)要描述:Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2547
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-28
  • 訪  問  量:1679

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞

ATCC® Number:CRL-2547™    Price:$338.00
Dsafety Level:1Shipesignations:Panc 10.05Depositors:EM JaffeeBioped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18 [50655]Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞Isolation:Isolation date: 1992Tumorigenic:YesOncogene:K-ras +Antigen Expression:MHC class I +; MHC class II -DNA Profile (STR):Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9,12 D5S818: 13 D7S820: 8,9 THO1: 6,9.3 TPOX: 11 vWA: 16Age:*****Gender:maleEthnicity:WhiteComments:Panc 10.05 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma. Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of 40%. [50655] The Panc 10.05 cell line was derived from the same patient as the PL45 cell line (ATCC CRL-2558).Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Temperature: 37.0°CSubculturing:Protocol:                        Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:19.2 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020derived from same individual:ATCC CRL-2558References:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲激情小视频| 国产99精品视频一区二区三区| 嘿咻嘿咻试看免费区国产| 91亚洲国产成人精品福利| 小秘书夹得真紧好爽h调视频| 天天躁日日躁狠狠躁va| 国产精品亚洲综合色区丝瓜| 日本熟妇人妻ⅹxxxx| 欧美 日韩 亚洲 熟女| 骚逼美女裸体大奶子被艹| 亚洲人成成无码网WWW| 成人小说亚洲一区二区三区| 激情人妻偷伦的在线视频| 麻豆国产精品va在线观看不卡 | 久久精品国产色蜜蜜麻豆国语版| 韩国黄色二级片| www亚洲无码| 扒开老师大腿猛进aaa片软件| 欧美成人网在线综合视频| 亚洲超碰碰中文字幕av| 欧美黑人又大又粗xxxxx| 熟女五十路开心久久伊人| 好男人网站www久久久| 男人桶插女人下面的视频| 老公的朋友跟我做完就不理我了 | 汤芳人体艺术| 神马久久久久久久久久久| 狂操欧美小骚逼| 99热在线这里只有精品| 小浪货腿张开水好多呀h| 狠狠噜天天噜日日噜av| 欧美性色欧美A在线墦放| 大鸡吧想日黑肥逼逼视频| 亚洲乱码国产乱码精品精剪| 久久久才是亚洲精品国产| 一本无码aⅴ久久久国产| 乖打开腿里面也要涂春药| 看到大鸡巴操骚逼的视频| 亚洲欧美国产日本一区二区| 18禁免费网站美女av| 和嫂子同居的日子|