CCL-141 Duck embryo 鴨子胚胎成纖維細(xì)胞

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CCL-141 Duck embryo 鴨子胚胎成纖維細(xì)胞 的詳細(xì)介紹

ATCC^® Number:

CCL-141™

Price:

$407.00

Designations:	Duck embryo
Depositors:	M Marcovici, J Prier, M Allen
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Anas platyrhynchus domesticus （duck, Pekin）
Morphology:	fibroblast
Source:	Organ: embryo
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance:	poliovirus 2
Age:	embryo
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:	Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. It is important to subculture the cells as soon as they become confluent, or else the cells will begin to die.1. Remove and discard culture medium.Briefly rinse the cell layer with 0.25% （w/v） Trypsin-053mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Do not attempt to break up cell clumpsAdd appropriate aliquots of the cell suspension to new culture vessels. Subction Ratio: 1:2 to 1:6.Incubate cultures at 37°C. Medium Renewal:* 1 to 2 times per week
References:	22136: Marcovici M, Prier JE. Enhancement of St. Louis arbovirus plaque formation by neutral red. J. Virol. 2: 178-181, 1968. PubMed: 4911850 26003: Melnick JL. Tissue culture techniques and their application to original isolation, growth, and assay of poliomyelitis and orphan viruses. Ann. N.Y. Acad. Sci. 61: 754-772, 1955. PubMed: 13340582 26004: Wolf K, et al. Duck viral enteritis: microtiter plate isolation and neutralization test using the duck embryo fibroblast cell line. Avian Dis. 18: 427-434, 1974. PubMed: 4368600 26005: Buynak EB, et al. Preparation and testing of duck embryo cell culture rubella vaccine. Am. J. Dis. Child. 118: 347-354, 1969. PubMed: 4307520 53245: Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741

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